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A μCT cross section and B 3D μCT reconstruction from automatic thresholding do not show the posterior incudal ligament in the bony wall recess. Arrows indicate the position of the invisible ligament. Pixel (and voxel) size is 8.5 × 8.5( × 8.5) μm. C–E ROI OPFOS cross sections from different orientations do show the ligament in the recess. F, G 3D OPFOS meshes. Voxel size is 0.97 × 0.97 × 2.5 μm. b bulla, I incus, L ligament, m malleus.
