Fig. 1.

Atgl−/− macrophages exhibit phenotype of alternatively activated macrophages (a) Wt and Atgl−/− macrophages were incubated in DMEM/10% LPDS, and cytokine secretion was determined by ELISA. Data represent mean values (n = 6) of two independent experiments ±SEM measured in triplicate. (b) Quantitative real-time PCR analyses of macrophages from Wt and Atgl−/− mice; Mcp2, macrophage chemoattractant protein 2; Sphk1, sphingosine kinase 1; Clec7a, C-type lectin domain family 7 member A; Mrc1, mannose receptor 1; Arg1, arginase 1; Ccl5/Rantes, C–C chemokine ligand 5; Gro1, chemokine (C-X-C motif) ligand 1. Data represent mean values (n = 4) of two independent experiments ± SEM performed in triplicate repeats. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001