Fig. 3.
Rg1 increases Akt phosphorylation and p70S6K signaling. HUVECs were untreated or treated with 150 nM Rg1 for various times as indicated. An equal amount of cell lysates (50 μg) was determined by immunoblotting. Akt and p70S6K activities were analyzed using anti-phospho-specific (p)-Akt and p-p70S6K antibodies. β-actin was also included as a loading control. The signal intensities were determined by densitometry and expressed as p-Akt and p-p70S6K relative to total Akt and p70S6K for each sample. Data are shown as mean ± SD of three independent experiments. *P < 0.05, difference with untreated control