Fig. 4.
Rg1 regulates HIF-1α expression via PI3K/Akt and p70S6K signaling. HUVECs were pretreated with a 10 μM LY294002 (LY) or b 10 nM rapamycin (Rp) before treated with Rg1 (150 nM) for 30 min. Equal amounts of protein (50 μg) were analyzed by immunoblotting using specific antibodies recognizing phospho (p)-Akt and p-p70S6K. The cells were also harvested 1 h after treatment to analyze HIF-1α expression. β-actin was included as a loading control. The signal intensity was determined by densitometry and expressed as p-Akt and p-p70S6K relative to total Akt and p70S6K, and HIF-1α and VEGF to β-actin for each sample. Data are shown as mean ± SD of three independent experiments. *P < 0.05, difference with untreated control