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. 1999 Feb;119(2):651–662. doi: 10.1104/pp.119.2.651

Figure 2.

Figure 2

DNA gel-blot analysis of two families segregating for the Lg3-O mutation in a B73 inbred line. The genomic DNA samples were digested with BamHI. Lane a, Lg3 plant 1678-7; lanes b (designated by arrows), seven progeny (four Lg3, three wild type) of the cross B73 wild type × plant 1678-7; lane c, plant 1678-8; lanes d (designated by arrows), seven progeny (four Lg3, three wild type) of the cross B73 wild type × plant 1678-8; lane e, B73 inbred tester. The DNA gel blot was probed with the BglII 1.6-kb fragment from the putative lg3 clone. The arrow marks a 10-kb fragment always present in Lg3-O heterozygotes but never present in wild-type siblings. The number scale on the right is in kilobases.