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RT-PCR analysis of Lg3 expression in wild-type and mutant tissues. cDNA from different tissues of wild-type and mutant plants was used for amplification of LG3 and ubiquitin sequences. Thirty cycles of PCR were used to amplify the LG3 and ubiquitin sequences. The PCR products were hybridized with the lg3 cDNA and a ubiquitin probe. dap, Days after pollination; Veg., vegetative.
