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RT-PCR analysis of Lg3 expression in sheath tissue from partially Lg3 revertant alleles. cDNA from sheath tissue from several Lg3 alleles and nonmutant siblings was used for amplification of Lg3 and ubiquitin sequences. Thirty cycles of PCR were used to amplify the Lg3 and ubiquitin sequences. The relative levels of the amplified products were similar at 20 cycles of PCR (data not shown). The PCR products were hybridized with the lg3 cDNA and a ubiquitin probe.
