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. 2004 Feb;24(3):1411–1425. doi: 10.1128/MCB.24.3.1411-1425.2004

FIG. 7.

FIG. 7.

Smad3 and TGF-β signaling regulate the transactivation function of IRF-7. (A) Smad3 and TGF-β stimulated the transcriptional activity of Gal4-IRF-7, and Smad3ΔC and Smad3C inhibited it. TGF-β enhanced the activity of Gal4-IRF-7. (B) Smad3 signaling and TGF-β signaling through the cytoplasmic TβRI/RII chimera, R(II-I)C, stimulated the transcriptional activity of IRF-7, and a dominant-negative TβRII, RII-DN, inhibited it. HepG2 cells were cotransfected with Gal4-IRF-7 and the luciferase reporter pFR-Luc and expression plasmids for Smads and TGF-β receptors. Luciferase activities were scored as described for Fig. 5C and D.