FIG. 7.

Stress-mediated silencing regulation by PNC1. (A) Northern blot analysis of steady-state PNC1 RNA levels from cells grown normally (at 30°C), at an elevated temperature (37°C), or in the presence of 0.02% MMS. The strains contained either an empty vector (pRS425) or the 2μm PNC1 plasmid (pJOE31). The ACT1 gene was used as a loading control. (B) Telomeric silencing spot assay for silencing of a URA3 reporter. WT (JJSy143), npt1Δ (JJSy137), and pnc1Δ (JJSy165) strains were spotted onto SC−Leu medium as a growth control, and SC−Leu+FOA medium to measure silencing. To partially inhibit silencing, 1 mM NAM was added. The strains were grown either at 30 or 37°C or in the presence of 0.02% MMS.