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Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pRS415 | CEN/ARS LEU2 shuttle vector | 12 |
| pRS425 | 2μm LEU2 shuttle vector | 12 |
| pRS424 | 2μm TRP1 shuttle vector | 12 |
| pSB765 | SIR2 in pRS425 | 10 |
| pJOE31 | PNC1 in pRS425 | |
| pJOE54 | PNC1 in pRS415 | |
| pJSS81 | NPT1 in pRS424 | 54 |
| pDM111 | pGEX-SIR2 | 68 |
| pJOE48 | pET-16b-PNC1 | |
| pCG64 | pET-16b-pnc1 (C167A) | |
| pJX43 | MSE-lacZ reporter plasmid | 73 |
| pAV124 | LacZ reporter plasmid lacking the MSE | 73 |
