TABLE 3.
PNC1 and NAM regulate HST1 activity
| Growth conditionsa
|
Activity of promoter-lacZ reporterb
|
Fold repressionc
|
||
|---|---|---|---|---|
| Plasmid | 5 mM NAM | −MSE | +MSE | |
| Empty | − | 25.89 (2.96) | 0.29 (0.15) | 89.3 |
| PNC1 | − | 26.86 (4.82) | 0.22 (0.09) | 122.1 |
| Empty | + | 16.02 (2.03) | 2.49 (0.15) | 6.4 |
| PNC1 | + | 27.36 (5.47) | 0.43 (0.22) | 63.6 |
a
Strain JB740 contained either the pRS425 empty vector or the pJOE31 2μm PNC1 plasmid. Each strain was grown with (+) or without (−) 5 mM NAM.
b
β-galactosidase units measured from whole-cell extracts from strains containing either pAV124 (−MSE) or pJX43 (+MSE). The reported units are averaged from three independent experiments. The average deviation calculated by Microsoft Excel is provided in parentheses.
c
Calculated as the β-GAL activity of pAV124 strains (−MSE) divided by the β-GAL activity of the pJX43 (+MSE) strains.