Figure 2.

Gel-retardation experiments with plastid extracts preincubated with various nucleotides. Experiments in A and B were done with radiolabeled LRP136 DNA fragments and plastid extracts that were preincubated with either 5.0 mm ATP, UTP, GTP, or CTP (A, lanes 2–5, respectively) or 1.0 mm ADP, UDP, GDP, CDP, or AMP (B, lanes 2–6, respectively). Plastid extracts were obtained from 4.5-d-old, dark-grown barley plants. The major complexes observed with the preincubation of plastid extracts without any exogenous nucleotides (control reaction, lanes 1) are labeled A, B, C, D, and E. Migration of the free probe (FP) is indicated.