Figure 3.

Gel-retardation experiments with plastid extracts preincubated with various concentrations of either ATP or ADP. Experiments were done with either radiolabeled LRP136 (A) or GT30 (B) DNA fragments and plastid extracts that were preincubated with ATP (A, B, lanes 2–4) or ADP (A, B, lanes 5–7). The concentration of ATP and ADP used is indicated above the lanes. Plastid extracts were obtained from 4.5-d-old, dark-grown barley seedlings. The major complexes observed in plastid extracts without exogenous nucleotides (control reaction, lanes 1) are labeled A, B, C, D, and E (A) and I, II, and III (B). Migration of the free probe (FP) is indicated and the binding activity of nonspecific-binding proteins to the GT30 probe is designated NS.