Figure 5.

Influence of ADP and ATP analogs on protein binding to the psbD BLRP. Plastid extracts were preincubated with ATP (lane 1), AMP-PNP (lanes 3–5), ATP-γ-S (lanes 6 and 7), ADP (lane 8), or ADP-β-S (lanes 9 and 10) prior to gel-shift assays using LRP136 as a probe. The concentration of the chemical used is indicated above the lanes. Binding experiments were done with plastid extracts obtained from 4.5-d-old, dark-grown barley plants. The major complexes observed without additions are labeled A, B, C, D, and E (control reaction, lane 1). Migration of the free probe (FP) is indicated.