Figure 6.

Gel-retardation experiments showing the effects of preincubation of plastid extracts with K252a. Gel-shift assays were carried out using radiolabeled LRP136 DNA fragment and plastid extracts obtained from 4.5-d-old, dark-grown barley plants. Before binding reactions, plastid extracts were preincubated either in the presence (lane 3) or absence (lane 4) of K252a (10 μm, final concentration), then further incubated with 1.0 mm ADP. The effect of the incubation of the plastid extracts with K252a alone was tested (lane 2). The major complexes observed with the preincubation of the extracts without additions are labeled A, B, C, D, and E (Control, lane 1). Migration of the free probe (FP) is indicated.