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. 2004 Feb;24(3):1245–1255. doi: 10.1128/MCB.24.3.1245-1255.2004

FIG. 1.

FIG. 1.

Expression pattern of CKIP-1 in C2C12 cells (A) Immunoblots for endogenous CKIP-1, myogenin, and p21 obtained with whole-cell extracts of C2C12 myoblasts cultured in GM and DM for up to 5 days. Loading was verified by immunoblotting for beta-tubulin. (B) Immunofluorescence for endogenous CKIP-1 and myogenin in C2C12 myoblasts and myotubes. C2C12 cells were cultured in GM (myoblasts) or in DM for 5 days (myotubes), and CKIP-1 (red) and myogenin (green) were visualized with a rabbit polyclonal anti-CKIP-1 antibody and a mouse monoclonal antimyogenin antibody as primary antibodies. The nuclei were stained with Hoechst reagent 33358. Staining was visualized by confocal microscopy. Bar, 5 μm. (C) Northern blot analysis for CKIP-1 transcripts in C2C12 myoblasts (GM) and myotubes after 5 days in DM (DM 5). Equal loading was verified with ethidium bromide-stained ribosomal 28S and 18S RNAs (EtBr).