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. 2004 Feb;24(3):1245–1255. doi: 10.1128/MCB.24.3.1245-1255.2004

FIG. 2.

FIG. 2.

Intracellular localization of CKIP-1 (A) (Top) Schematic presentation of wild-type and mutant CKIP-1 expressed in C2C12 cells. The mutant CKIP-1ΔPH was obtained by deleting the first 146 amino acids of CKIP-1, corresponding to the PH domain. (Bottom) Immunolocalization of wild-type and mutant CKIP-1 proteins in growing C2C12 cells. C2C12 cells were transfected with pcDNA3 CKIP-1 (CKIP-1) or pcDNA3-CKIP-1ΔPH (CKIP-1ΔPH) and cultured in GM for a further 18 h. After fixation, cells were stained with a rabbit anti-CKIP-1 polyclonal antibody and visualized by confocal microscopy. Bar, 5 μm. (B) Distribution of wild-type and mutant CKIP-1 proteins in the nuclear (N), soluble (S100), and particulate (P100) fractions. Fractions were prepared from C2C12 cells expressing wild-type CKIP-1 or CKIP-ΔPH cultured in GM for 18 h following transfection. A 10-μg portion of each fraction was analyzed by immunoblotting using the anti-CKIP-1 polyclonal antibody.