TABLE 1.
Analysis of embryos from Oct-1+/− crossesa
| Gestational age | Total no. of embryos | No. of resorbed embryos | No. genotyped | No. (%) of embryos
|
||||
|---|---|---|---|---|---|---|---|---|
| +/+ | +/− | −/− | −/− runted | −/− dead | ||||
| E13.5 | 211 | 20 | 191 | 47 (24.6) | 110 (57.6) | 20 (10.5) | 7 (3.6) | 7 (3.6) |
| E18.5 | 30 | 5 | 24 | 6 (25) | 17 (70.8) | 2 (8.3) | 1 (4.2) | 1 (4.2) |
| E20.5 (pups) | 380 | 12 | 368 | 145 (39.4) | 223 (60.6) | 0 (0) | 0 (0) | 0 (0) |
a
Genotypes of resorbed embryos were not available. Nonresorbed embryos and newborn pups were genotyped by PCR and Southern blotting with yolk sac and tail DNA as described in the text. The −/− group included only embryos which were alive and anatomically normal at the time of dissection, whereas the −/− runted group consisted of embryos which were visibly smaller and paler but alive.