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Mutation of p53Ser18 does not affect basal or ionizing radiation-induced levels of p53 protein. Western analysis of extracts from irradiated (20 Gy) and nonirradiated thymocytes. Extracts were prepared at 0 h (nonirradiated) or 8 h after ionizing radiation treatment. α-Tubulin was used as a control for the Western blot. WT, wild type.
