TABLE 4.
Rates of chromosome missegregation events in RSC componentsa
| Genotype | Rate of:
|
Total colonies | |
|---|---|---|---|
| Chromosome loss (1:0 events) | Nondisjunction (2:0 events) | ||
| +/+ | 8.7 × 10−5 (1.0) | 8.7 × 10−5 (1.0) | 22,800 |
| rsc1Δ rsc1Δ | 5.4 × 10−5 (0.6) | 1.1 × 10−4 (1.3) | 18,450 |
| rsc2Δ rsc2Δ | 8.8 × 10−4 (10.1) | 1.7 × 10−3 (19.5) | 19,320 |
| npl6Δ npl6Δ | 2.1 × 10−4 (2.4) | 1.6 × 10−4 (1.8) | 18,072 |
| rtt102Δ rtt102Δ | 4.4 × 10−4 (5.1) | 1.8 × 10−4 (2.1) | 27,072 |
a
Mutants were plated to single colonies, and visual sectoring phenotypes were scored. Colonies scored as half-sectored were ≥50% red (23). Strains used were YPH982, YKB408, YKB409, YKB410, and YKB411. Chromosome loss or 1:0 events were scored as colonies that were half red and half pink; nondisjunction or 2:0 events were scored as colonies that were half red and half white. Numbers in parentheses are factors of increase in rates of missegregation events above wild-type rates.