FIG. 2.

Degradation of CLB2 mRNA in the nme1-P6 mutant. Wild-type (MES111-140) and nme1-P6 mutant (MES111-P6) strains were arrested in hydroxyurea for 2 h at 25°C and then shifted to 37°C for 1 h. Cells were then washed to remove the hydroxyurea, resuspended in fresh medium, and maintained at 37°C for 60 min. The transcriptional inhibitor 1,10-phenanthroline was added, and RNA was harvested at the indicated times (see Materials and Methods). The CLB2 and ACT1 mRNAs are shown.