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In vitro cleavage of the CLB2 5′-UTR by RNase MRP. (A) Internally labeled RNA substrates containing the rRNA A3 cleavage site (positive control), the CLB2 5′-UTR or the 5.8S rRNA (noncleaved control RNA) were generated. Highly pure yeast RNase MRP was purified with the TAP tag protocol and used in a standard RNase MRP in vitro assay (3, 22). (B) The schematic indicates the source of the RNA substrates and the sizes of the RNAs.
