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Both RNase MRP and Xrn1 participate in CLB2 mRNA degradation. The relevant genotypes of the yeast strains used are indicated; complete genotypes can be found in Table 1 (wild-type, MES111-140; nme1-P6, MES11-P6; xrn1Δ, TLG105; xrn1Δ nme1-P6, TLG105-P6; clb2Δ, YJA103). Cells were grown at 24°C, and equal amounts of RNA (based on both rRNA staining [bottom panel] and subsequent ACT1 mRNA analysis) were subjected to Northern analysis for the CLB2 mRNA. The CLB2 mRNA is indicated.
