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Identification of in vivo-generated RNase MRP products. Primer extension was performed on the same RNA used in Fig. 6 (34), with an oligonucleotide that hybridizes from positions −240 to −218 (from the CLB2 translational start). Previously identified full-length and novel xrn1Δ-specific ends are indicated (14). Lanes 1 to 4, wild-type, xrn1Δ, nme1-P6, and xrn1Δ nme1-P6, respectively. A sequencing reaction generated with the same primer on a plasmid version of the CLB2 gene is shown.
