Table 3.

Peptide sequences and in vitro determined K _D values for the three different MHC molecules PPP (SLA-1*0401) (top), PHP (pα₁hα₂pα₃) (middle), and HPP (hα₁pα₂pα₃) (bottom), respectively

Peptide #	Sequence	Affinity (K D, nM)
PPP
1	HSNASTLLY	452
2	SSMNSFLLY	192
3	YSAEALLPY	202
4	MTFPVSLEY	15
5	MSSAAHLLY	103
6	STFATVLEY	32
7	MTAASYARY	16
8	HTSALSLGY	163
9	ASYQFQLPY	8
10	YANMWSLMY	386
11	STYQPLPLY	38
12	HTAAPWGSY	Non-binder
13	ITMVNSLTY	169
14	ATAAATEAY	5
PHP
1	HSNASTLLY	5
2	SSMNSFLLY	2
4	MTFPVSLEY	1
5	MSSAAHLLY	1
6	STFATVLEY	3
9	ASYQFQLPY	1
13	ITMVNSLTY	2
15	STMPLSWMY	1
16	HMMAVTLFY	11
17	KTFEWGVFY	6
18	ATNNLGFMY	1
19	ATATWFQYY	3
20	ITMVNSLTY	2
HPP
2	SSMNSFLLY	Non-binder
4	MTFPVSLEY	Non-binder
6	STFATVLEY	Non-binder
21	FSFGGFTFK	Non-binder
22	KVFDKSLLY	Non-binder
23	ATFSVPMEK	Non-binder
24	RVMPVFAFK	17
25	AVYSSSMVK	127
26	AVARPFFAK	231
27	GSYFSGFYK	Non-binder
28	LTFLHTLYK	Non-binder
29	MTMRRRLFK	Non-binder

The NetMHCpan percentage of success for predicting binders for the PPP, PHP, and HPP heavy chains were 93%, 100%, and 33%, respectively