Figure 5.
Slc1 interacts with TARP in C. trachomatis elementary bodies. Immunoprecipitation of TARP (top blot) from EB lysates pulled down Slc1 (bottom blot) indicating interaction of the chaperone with TARP in Chlamydia EBs. Lanes from left to right are molecular weight markers (MW), lysate (Lys), wash (W) and elution (E) fractions. Anti-TARP blot was stripped and reprobed with anti-Slc1 antibody. The 103 kDa TARP and the 18 kDa Slc1 bands are indicated by the arrow and arrowheads, respectively. Asterisks indicate the background band to differentiate it from the Slc1 band.