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. Author manuscript; available in PMC: 2012 Oct 25.
Published in final edited form as: Biochemistry. 2011 Sep 28;50(42):9135–9147. doi: 10.1021/bi2012178

Figure 1.

Figure 1

TLC comparison of crude lipid extracts from M. tuberculosis H37Rv. Purified standards or crude lipids that were obtained under various conditions were spotted and developed in two solvents in the same direction. Standards, samples and the conditions that they were obtained with (if known) are indicated. Lane 1-SL-I standard from the CSU TB materials research contract (GAS broth; chloroform/methanol extraction (C/M); lane 2-trehalose dimycolate standard from Sigma-Aldrich; lane 3-purified major SL (GAS broth; C/M); lane 4-crude lipids (7H10 plates, hexane-decylamine extract (HD); lanes 5 and 6-crude lipids from different cultures (7H9 broth, HD); lane 7-crude lipids (Wong-Weinzirl broth, HD); lane 8-crude lipids (7H10 plates, C/M), and lane 9-crude lipids (7H9 broth, C/M). The average Rfs of the SL families are indicated as well as the origin and the farthest solvent front.