TABLE 4.
Some primers used in the characterization of the SSU rRNA, HSP70, and actin genes of various Cryptosporidium spp.
Gene | Primers
|
Amplicon size (bp) | Usage | Reference | |
---|---|---|---|---|---|
Name | Sequence (5′ to 3′) | ||||
SSU rRNA | SSU-F1 | AACCTGGTTGATCCTGCCAGTAGTC | ∼1,750 | Amplify the full rRNA gene of most eukaryotic organisms | 256 |
SSU-R1 | TGATCCTTCTGCAGGTTCACCTACG | ||||
SSU rRNA | SSU-F2 | TTCTAGAGCTAATACATGCG | ∼1,325 | Both sets of primers are Cryptosporidium specific and can be used in nested PCR | 246, 256 |
SSU-R2 | CCCATTTCCTTCGAAACAGGA | ||||
SSU-F3 | GGAAGGGTTGTATTTATTAGATAAAG | ∼820 | |||
SSU-R3 | AAGGAGTAAGGAACAACCTCCA | ||||
HSP70 | HSP-F1 | ATGTCTGAAGGTCCAGCTATTGGTATTGA | ∼2,010 | Both sets of primers amplify most apicomplexan parasites and are used in nested PCR | 213 |
HSP-R1 | TTAGTCGACCTCTTCAACAGTTGG | ||||
HSP-F2 | TA/CTTCATG/CTGTTGGTGTATGGAGAAA | ∼1,950 | |||
HSP-R2 | CAACAGTTGGACCATTAGATCC | ||||
Actin | Act-F1 | ATGA/GGA/TGAAGAAGA/TAA/GC/TA/TCAAGC | ∼1,095 | Both sets of primers amplify most apicomplexan parasites and are used in nested PCR | 211 |
Act-R1 | AGAAG/ACAC/TTTTCTGTGT/GACAAT | ||||
Act-F2 | CAAGCA/TTTG/AGTTGTTGAT/CAA | ∼1,066 | |||
Act-R2 | TTTCTGTGT/GACAATA/TG/CA/TTGG |