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. 2004 Jan;17(1):72–97. doi: 10.1128/CMR.17.1.72-97.2004

TABLE 4.

Some primers used in the characterization of the SSU rRNA, HSP70, and actin genes of various Cryptosporidium spp.

Gene Primers
Amplicon size (bp) Usage Reference
Name Sequence (5′ to 3′)
SSU rRNA SSU-F1 AACCTGGTTGATCCTGCCAGTAGTC ∼1,750 Amplify the full rRNA gene of most eukaryotic organisms 256
SSU-R1 TGATCCTTCTGCAGGTTCACCTACG
SSU rRNA SSU-F2 TTCTAGAGCTAATACATGCG ∼1,325 Both sets of primers are Cryptosporidium specific and can be used in nested PCR 246, 256
SSU-R2 CCCATTTCCTTCGAAACAGGA
SSU-F3 GGAAGGGTTGTATTTATTAGATAAAG ∼820
SSU-R3 AAGGAGTAAGGAACAACCTCCA
HSP70 HSP-F1 ATGTCTGAAGGTCCAGCTATTGGTATTGA ∼2,010 Both sets of primers amplify most apicomplexan parasites and are used in nested PCR 213
HSP-R1 TTAGTCGACCTCTTCAACAGTTGG
HSP-F2 TA/CTTCATG/CTGTTGGTGTATGGAGAAA ∼1,950
HSP-R2 CAACAGTTGGACCATTAGATCC
Actin Act-F1 ATGA/GGA/TGAAGAAGA/TAA/GC/TA/TCAAGC ∼1,095 Both sets of primers amplify most apicomplexan parasites and are used in nested PCR 211
Act-R1 AGAAG/ACAC/TTTTCTGTGT/GACAAT
Act-F2 CAAGCA/TTTG/AGTTGTTGAT/CAA ∼1,066
Act-R2 TTTCTGTGT/GACAATA/TG/CA/TTGG
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