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. 2004 Jan;17(1):72–97. doi: 10.1128/CMR.17.1.72-97.2004

TABLE 6.

Detection of Cryptosporidium in natural water by PCR-based molecular techniques

Methoda Gene target Vol of water (liters) No. of positive samples/total no. of samples Species or genotypeb Reference
CC-PCR HSP70 10 Source water: 6/122 7 sequence types, probably from C. parvum, C. hominis, and mouse genotype 51
Backwash water: 9/121
IMS-nested PCR-RFLP SSU rRNA 189-224 (IMS done on Percoll-sucrose concentrates) Storm water: 27/29 12 species/genotypes, all from wildlife 246
IMS-nested PCR TRAP-C2 500-1,000 Surface and finished water: 11/214 C. parvum 113
IMS-PCR SSU rRNA and TRAP-C2 500 River water and sewage effluent: 2/10 C. parvum 114
IMS-nested PCR-RFLP SSU rRNA Surface water: 10-63.1 Wastewater: 0.01-0.50 Surface water: 25/55 Raw wastewater: 12/49 Surface water: C. andersoni (5), C. hominis (10), C. parvum (19), C. baileyi (1) 253
Wastewater: C. andersoni (8), C. hominis (1), C. parvum (1), C. canis (1), C. muris (1), C. felis (1), cervine genotype (1)
RT-PCR HSP70 2.5-10 River water: 2/6 C. parvum (1); C. parvum and C. meleagridis (1) 95
IMS-nested PCR SSU rRNA 40-80 Surface water: 7/78 C. parvum? (3), C. andersoni (4), C. baileyi? (1) 92
IMS-nested PCR SSU rRNA 2 or 20 Surface water: 24/60 Wastewater: 6/8 C. parvum (6) and C. hominis (4), C. muris (6), C. andersoni (3), C. baileyi (1), 3 new genotypes (3), dinoflagellates (7) 238
a

Methods detect only C. parvum, C. hominis, C. meleagridis, and closely related parasites. CC-PCR, cell culture PCR; IMS, immunomagnetic separation.

b

Number of samples where the species was detected is given in parentheses.

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