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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Cancer Prev Res (Phila). 2011 Sep 7;4(10):1536–1545. doi: 10.1158/1940-6207.CAPR-11-0120

Fig. 2.

Fig. 2

Kinetics of the time-dependent inhibition of COX-2 by compound 7. The purified mCOX-2 enzyme was reconstituted with heme and pre-incubated with the inhibitor at 37 °C for various times (0, 0.5, 1, 3, 5, 10, 15, 30, 45, 60 and 120 min) prior to the addition of the substrate (50 μM). (A) Time-dependent inhibition of COX-2 by compound 7 at the indicated concentrations. (B) Secondary plot of kobs versus inhibitor concentration used to generate values for KI, k2 and k−2 using eqs 1 and 2 (see text).