Fig. 6.

In vivo uptake statistics in tumors. (A) Female nude mice bearing 1483 HNSCC and HCT116 tumors were dosed with compound ¹⁸F-7 (∼350 μCi, r.o.). The animals were imaged at 3 h post-injection in the microPET/CT Focus 220 instrument. After imaging, the animals were sacrificed by isoflurane overdose. The 1483 HNSCC and HCT116 tumors and leg muscles were removed and weighed, and radioactivity associated with each tissue was counted with a well gamma counter. The plot shows the increased radiotracer (Bq/g) in COX-2-expressing 1483 tumors versus COX-2-negative HCT116 tumors (n = 3, p = 0.0004). (B) Nude mice bearing 1483 xenograft tumors were pre-dosed with vehicle or with celecoxib (20 mg/kg, i.p.) immediately prior to ¹⁸F-7 dosing (∼350 μCi, r.o.) and imaged at 3 hrs post-injection. After imaging, the animals were sacrificed by isoflurane overdose, and tissues were removed and counted as in 6A. The plot shows the fold increase of radiotracer in the 1483/vehicle tumor to muscle ratio compare to the 1483/celecoxib tumor to muscle ratio (n = 4, p = 0.0004).