Fig. 6.

Western blots of chicken PML-L and PML-S proteins detected in different cell types. Each of the 4 cell lines was analyzed using the 2 anti-chicken PML antibodies designed for this study (see Materials and Methods). One hundred micrograms of total protein were analyzed for each cell line. For PML-L, DT40 exhibited a protein at 150 kDa, while OU2 and CEF001 exhibited proteins at both 150 kDa and 70 kDa. For PML-S, DT40 and OU2 showed a protein at 48 kDa. Interestingly, no PML-S proteins were detected in CEF001 and neither PML-L nor PML-S proteins were detected in DF-1 even when the concentration of protein was increased from 100 μg to 160 μg.