Table 3.
. Immunofluorescence analysis of PML and TRF2 proteins in nuclei of chicken cells
| Proteins | Immunofluorescence analysis | CEF001 | DF-1 | OU2 | DT40 |
|---|---|---|---|---|---|
| PML-L | positive cells (%) | 94 | 48 | 99 | 92 |
| signals/cell average ± S.D. | 12.8 ± 5.6 | 2.6 ± 1.9 | 28.7 ± 7.7 | 4.4 ± 2.5 | |
| signals/cell range (low–high) | 1–29 | 1–10 | 3–101 | 1–15 | |
| PML-S | positive cells (%) | 94 | 42 | 96 | 47 |
| signals/cell average ± S.D. | 7.6 ± 4.0 | 2.6 ± 1.5 | 16.1 ± 6.1 | 2.5 ± 1.3 | |
| signals/cell range (low–high) | 1–27 | 1–9 | 2–45 | 1–7 | |
| TRF2 | positive cells (%) | 100 | 100 | 98 | 99 |
| signals/cell average ± S.D. | 29.8 ± 7.0 | 29.8 ± 6.5 | 32.8 ± 6.7 | 16.7 ± 4.8 | |
| signals/cell range (low–high) | 14–83 | 8–64 | 8–64 | 4–34 | |
In addition to the percentage of positive cells for the protein, the number of protein signals (dots) was determined. Values are the average signals/cell ± standard deviation (S.D.) and the range (low–high) of signals/cell. At least 120 cells were counted for each cell line and protein assayed.