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Western blot of proteins (20 μg/lane) extracted from embryos that were exposed at 25°C for 2 h (lane 1) or at 37°C for 15 min (lane 2), 30 min (lane 3), 60 min (lane 4), or 120 min (lane 5). For recovery the embryos that were exposed for 2 h at 37°C were incubated at 25°C for an additional 4 h (lane 6), 6 h (lane 7), 8 h (lane 8), or 24 h (lane 9). The blot was immunodecorated with the polyclonal antibodies raised against recombinant wFKBP73 (anti 73).
