TABLE 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| 3841 | Rhizobium leguminosarum bv. viciae; Smr derivative of strain 300 | 15 |
| RU1327 | 3841 aldA::TnB20; Smr Kmr | 1 |
| RU1422 | 3841 aldR::Ω; Smr Spr | This study |
| Cosmid, pRU3135 | Cosmid containing aldAR Tcr | 1 |
| Plasmids | ||
| pRU640 | 1.45-kb PCR product (with primers P199 and P200) of aldA in pCR2.1-TOPO; Apr/Kmr | This study |
| pRU679 | 700-bp PCR product (with primers P219 and P220) of aldA-aldR intergenic region in pCR 2.1-TOPO; Apr Kmr | This study |
| pRU693 | 3-kb aldA-aldR SalI fragment from pRU3135 in pSK(−); Apr | This study |
| pRU701 | 0.7-kb aldA-aldR intergenic region in pSK(−); Apr | This study |
| pRU708 | 1.45-kb SacI/KpnI fragment from pRU640 in pTR101; Tetr Apr | This study |
| pRU730 | aldR::gusA fusion in pJP2; Tcr | This study |
| pRU731 | aldA::gusA fusion in pJP2; Tcr | This study |
| pRU734 | Ω cloned in pRU693 (aldR); Apr Spr | This study |
| pRU735 | aldR::Ω cloned in pJQ200SK; Gmr Spr | This study |
| pRU876 | 1-kb PCR product (with primers P287 and P289) of aldA-aldR intergenic region in pCR2.1-TOPO; Apr Kmr | This study |
| pRU877 | gusA in pK19mob; Kmr | This study |
| pRU882 | AldR::gusA fusion in pRU877; Kmr | This study |
| pRU883 | AldA::gusA fusion in pRU877; Kmr | This study |
| pRU889 | 1.9-kb PCR product (primers P280 and P281) of aldA-aldR in pTR101; Tcr | This study |
| pHP45Ω | pBR322 derivative carrying Ω; pHP45 replicon; Apr Spr | 27 |
| pJP2 | Wide-host-range stable gusA transcriptional promoter probe vector; Tcr | 26 |
| pJQ200SK | P15A origin from pACYC184; GmrlacZ sacB traJ | 28 |
| pK19mob | pUC19-derived mobilizable integration vector; Nmr | 30 |
| pCR2.1-TOPO | TA PCR cloning vector, f1 origin, ColE1 replicon; Apr KmrlacZ | Invitrogen |
| pTR101 | Wide-host-range stable mobilizable P-group cloning vector, RK2 derivative; Tcr | 38 |
| pBluescript II SK(−) | Phagemid, pUC19 derivative, f1 origin of replication, ColE1 replicon; Apr | Stratagene |