TABLE 2.
l-Alanine dehydrogenase activities of various strains grown on different carbon sources
| Carbon source(s) |
l-Alanine dehydrogenase activity (μmol min−1 mg of protein−1)a in:
|
||||
|---|---|---|---|---|---|
| 3841 (WT) | RU1327 (ΔaldA) | RU1422 (ΔaldR) | 3841/pTR101 | 3841/pRU889 | |
| Glc-NH3 | ND | ND | − | − | 0.034 ± 0.013 |
| Succ-NH3 | 0.074 ± 0.014 | ND | − | − | − |
| Glc-Ala | 0.494 ± 0.015 | ND | ND | 0.286 ± 0.054 | 3.96 ± 0.450 |
| Ala | 0.770 ± 0.022 | − | − | 0.645 ± 0.101 | 5.8 ± 0.268 |
| Mal-NH3 | 0.084 ± 0.003 | − | − | 0.136 ± 0.02 | 7.1 ± 0.454 |
| Pyr-NH3 | 0.079 ± 0.004 | − | − | − | − |
| Glc-Succ-NH3 | 0.066 ± 0.006 | − | − | − | − |
| Bacteroids | 0.09b | − | − | 0.069 | 0.463 |
a
All carbon and nitrogen sources were at 10 mM except when alanine (20 mM) was used as the sole carbon source. Activities from at least three independent cultures ± SEMs are shown. Bacteroid activities are means from two independent harvests. Abbreviations: Glc, glucose; Succ, succinate; Ala, alanine; Mal, malate; Pyr, pyruvate; ND, no detectable activity; −, not tested; WT, wild type.
b
The activity of AldA in strain 3841 is from previous work (1).