TABLE 3.
Transcriptional activities of aldA and aldR fusions in strains grown on various carbon and nitrogen sources
| Strain and fusion | GusA activity (nmol min−1 mg of protein−1)a on:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Glc-NH3 | Mal-NH3 | Succ-NH3 | Pyr-NH3 | Ala-Glc | Ala | Glu-Glc | Glu | |
| 3841/pRU730 (aldR::gusA in pJP2) | 163 ± 20 | − | 325 ± 64 | − | 555 ± 145 | 1,144 ± 244 | − | − |
| 3841/pRU731 (aldA::gusA in pJP2) | 104 ± 9 | − | 227 ± 31 | − | 349 ± 100 | 757 ± 152 | − | − |
| 3841/pRU882 (aldR::gusA genome) | 112 ± 18 | 237 ± 55 | 220 ± 10 | 310 ± 8 | 519 ± 40 | 1,142 ± 46 | 140 ± 1 | 366 ± 22 |
| 3841/pRU883 (aldA::gusA genome) | 124 ± 8 | 194 ± 40 | 184 ± 3 | 219 ± 9 | 598 ± 30 | 1,082 ± 29 | 109 ± 3.11 | 318 ± 3 |
| RU1422/pRU730 (aldR::gusA in pJP2) | ND | − | ND | − | ND | ND | − | − |
| RU1422/pRU731 (aldA::gusA in pJP2) | ND | − | ND | − | ND | ND | − | − |
a
Values shown are means ± SEMs. Strains carrying pJP2 fusions (pRU730 and pRU731) were assayed in at least 15 independent cultures, while strains carrying integrated fusions (pRU882 and pRU883) were assayed in at least 3 independent cultures. Strain 3841 containing pJP2 without an insert had no detectable GusA activity. Abbreviations: Glc, glucose; Mal, malate; Succ, succinate; Ala, alanine; Pyr, pyruvate; Glu, glutamate; ND, no detectable activity; −, not tested.