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Analysis of rep2037 from free-living M. loti NZP2037 and nodule extracts containing NZP2037 from L. corniculatus by the gel retardation assay. Lane 1, nodule extract; lane 2, M loti NZP2037 extract. Each assay mixture contained 1 ng of radiolabeled 59-bp RBD probe, 2 μg of protein from extracts of nodule or free-living bacteria, and 1 μg of poly(dI-dC) and poly(dA-dT) competitor DNA.
