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. 2004 Feb;186(3):811–817. doi: 10.1128/JB.186.3.811-817.2004

FIG. 3.

FIG. 3.

Analysis of complex formation with the 32P-labeled 59-bp RBD probe by using protein extracts from nodules formed with M. loti NZP2037 and M. loti symbiotic mutants. Lane 1, Lotus and PN 236; lane 2, Lotus and PN 238; lane 3, Lotus and PN 239; lane 4, Lotus and NZP2037. Each assay mixture contained 1 ng of radiolabeled 59-bp RBD probe, 2 μg of protein extract, and 1 μg of poly(dI-dC) and poly(dA-dT) competitor DNA.