Skip to main content
. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Biochemistry. 2011 Oct 18;50(45):9708–9723. doi: 10.1021/bi201277j

Figure 2. Size exclusion chromatography of wild-type and BC domain deletion RePC.

Figure 2

Representative chromatograms for wild-type RePC (wt RePC; long dashed line) and the BC domain deletion of RePC (ΔBC). The elution profile of ΔBC RePC is unaffected by the presence (short dashed line) or absence (solid line) of acetyl-CoA. INSET: Standard curve (open circles) and predicted Kav for the ΔBC monomer, dimer and tetramer (open squares). The measured Kav for wild-type and ΔBC is indicated (closed circles), corresponding to a dimer for ΔBC RePC (MW = 150 kDa) and a tetramer for wild-type RePC (MW = 500 kDa).