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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Cell Tissue Res. 2011 May 3;344(3):511–517. doi: 10.1007/s00441-011-1167-9

Fig. 2.

Fig. 2

Abnormal Osx and Col1a1 expression and TGFβ and BMP signaling in shFbnOP cells. a q-PCR quantification of Osterix (Osx) and collagen I (Col1a1) transcripts in shScrOP, shFbn1OP and shFbn2OP cells. b Collagen I immunoblots of protein extracts from shScrOP, shFbn1OP and shFbn2OP; the same amount of total proteins (25 µg) was loaded in each of the samples. c Osx promoter-driven luciferase activity in shScrOP, shFbn1OP and shFbn2OP cells expressed as average fold induction over control±SD. d pSmad2 immunoblots of protein extracts from shFbn1OP (left), shFbn2OP (right) and the control shScrOP cells lines with histograms summarizing the average pSmad2/Smad2 ratios±SD. e pSmad1/5/8 immunoblots of protein extracts from shScrOP cells and shFbn1OP or shFbn2OP cells with histograms summarizing the relative ratios of pSmad1/5/8 over β-actin±SD. Asterisks in relevant panels indicate statistical significance (p<0.05)