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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Cell Tissue Res. 2011 May 3;344(3):511–517. doi: 10.1007/s00441-011-1167-9

Fig. 3.

Fig. 3

shFbnOP cells exhibit abnormal proliferation and Runx2. activity. a PCNA immunoblots of protein extracts from shScrOP, shFbn1OP, and shFbn2OP cells collected at the indicated time points; histograms summarize the ratios between PCNA and β-actin. b PCNA immunoblots of protein extracts from day 2 shFbn2OP cells cultured with or without BMP2 and illustrative examples of alizarin red-stained day 4 shFbn2OP cell cultured with or without BMP2. c q-PCR analysis of Runx2 mRNA levels in shScrOP, shFbn1OP and shFbn2OP cells collected at the indicated time points; average fold changes are reported±SD. d Runx2 and β-actin immunoblots of protein extracts from shScrOP, shFbn1OP and shFbn2OP cells lines collected at the indicated time points. e Runx2 promoter-driven luciferase activity in shScrOP, shFbn1OP and shFbn2OP cells expressed as average fold induction over control±SD. Asterisks in relevant panels indicate statistical significance (p<0.05)