Fig. 3.

xCITED2 induced neurogenesis in Xenopus Animal cap. (A) Expression level of HA-xCITED2 was confirmed by western-blot analysis. 1 ng of HA-xCITED2 RNAs were injected at 1 cell stage embryo and harvested at stage 12. Immuno-bloting was performed using anti-HA probe. (B) 1 ng of xCITED2 RNAs were injected at 1 cell stage and dissected animal cap at stage 8 and incubated in animal cap media until stage 12 or stage 24 (C). RT-PCR was performed to analyze various genes expression. ODC was used as loading control, ventral marker: Xvent1/2, GATA2, PV.1, endoderm marker: mixer and Edd, mesoderm marker: Xbra, Actin, organizer marker: chordin and neural marker: Zic3, NCAM, Krox20.