Fig. 4.

Analysis of neurite length of rat hippocampal neuronal cells transfected with the indicated plasmids and/or siRNAs. Rat E17 primary dissociated hippocampal neuronal cells were cultured and the indicated plasmids and/or siRNAs were transiently transfected on the fourth day after seeding. Cells were fixed for microscopic analysis after incubation for 7 more days and analyzed for total neurite length as explained in Fig. 2. ns means no significant difference between the indicated groups by ANOVA test (p>0.05). Lanes 2, 3, 5, 6 and 7, but not lanes 4, showed statistically significant differences compared to the control, lane 1 (p<0.05).