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Association of H. pylori RecA with the bacterial membrane. H. pylori wild-type strain P1 and the isogenic eno and hp158 mutants were separated into total membrane fractions and cytoplasmic fractions. Immunoblotting with AK263 reveals not only a RecA association with the membranes but also a localization in the cytoplasm. Equal amounts of membrane fractions and cytoplasmic fractions were loaded in each lane.
