Fig. 3.

The effect of BF on apoptotic cell death after SCI. Spinal cord tissues from 1 d after injury were processed for TUNEL staining as described in the Method section (n=5/group). (A) TUNEL-positive cells in the GM at 1 d after injury. Representative images were from the sections selected 1 mm rostral to the lesion epicenter. Bar, 50µm. (B) Quantitative analysis of TUNEL-positive cells in the GM at 1 d after injury. Serial transverse sections (10µm thickness) were collected every 100 µm from 2 mm rostral to 2 mm caudal to the lesion epicenter (total 40 sections for neurons). Data represent mean±SD obtained from five separate experiments. ^*p<0.05.