FIG. 1.

(A) Effect of salt and temperature on growth of RG60. RG60 (ftsE400::kan) growing in LB with 1% NaCl at 30°C was subcultured into LB with 1% (circles) or no (squares) NaCl at 30°C (open symbols) or 37°C (closed symbols). The inset shows a phase-contrast micrograph of cells harvested at the time indicated by the arrow from the cultures growing with salt. (B) Localization of FtsN. Cells of wild type (MG1655) or RG60 in exponential growth in LB with 1% NaCl at 30°C were fixed, and FtsN was visualized by immunofluorescence microscopy. Arrows point to septal localization of FtsN.