TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant features | Source or reference |
|---|---|---|
| Strains | ||
| MG1655 | Wild type | Lab collection |
| DHB4 | Wild type with F′ lacIq | 5 |
| EC549 | ftsI::TnphoA (Kanr)/pBAD33-ftsI | 38 |
| JOE170 | ftsQ::TnphoA (Kanr)/pBAD33-ftsQ | 8 |
| JOE563 | ftsK::cat/pBAD42-ftsK | 7 |
| JMG265 | ftsL::TnphoA (Kanr)/pBAD33-ftsL | 12 |
| PS223 | W3110 zipA1(Ts) | 28 |
| RG60 | MG1655 ftsE::kan | 10 |
| EC1063 | MG1655 Δ(attL-lom)::(kan lacIq P204-ftsX-gfp) | This study |
| EC1065 | MG1655 Δ(attL-lom)::(kan lacIq P206-gfp-ftsX) | This study |
| EC1116 | MG1655 attφ80::pDSW533 (P206-gfp-ftsX) | This study |
| EC1152 | EC1116 ftsA12(Ts) leu::Tn10 | This study |
| EC1158 | EC1116 ftsZ84(Ts) | This study |
| EC1159 | EC1116 ftsW::kan/pBAD33-ftsW | This study |
| EC1179 | JOE170 attφ80::pDSW533 (P206-gfp-ftsX) | This study |
| EC1180 | JMG265 attφ80::pDSW533 (P206-gfp-ftsX) | This study |
| EC1181 | EC549 attφ80::pDSW533 (P206-gfp-ftsX) | This study |
| EC1295 | JOE563 Δ(attL-lom)::(kan P204-ftsX-gfp) | This study |
| EC1335 | MG1655 ftsE::kan/pBAD33-ftsEX | This study |
| EC1340 | PS223 attφ80::pDSW533 (P206-gfp-ftsX) | This study |
| EC1363 | EC1335 Δ(attL-lom)::(bla lacIq P206-ftsA-gfp) | This study |
| EC1366 | EC1335 Δ(attL-lom)::(bla lacIq P204-gfp-ftsI) | This study |
| EC1386 | EC1335/pCH205 [Plac-ftsK(1-266)-gfp] | This study and ref. 18 |
| EC1391 | EC1335 Δ(attL-lom)::(bla lacIq P206-zipA-gfp) | This study |
| EC1392 | EC1335 Δ(attL-lom)::(bla lacIq P206-gfp-ftsQ) | This study |
| Plasmids | ||
| pBAD33 | Arabinose regulation (PBAD), p15A ori, Camr | 15 |
| pDSW206 | P206 promoter, ColE1 ori, Ampr | 38 |
| pDSW209 | gfp-fusion vector, P206 promoter, ColE1 ori, Ampr | 38 |
| pDSW210 | gfp-fusion vector, P206 promoter, ColE1 ori, Ampr | 38 |
| pDSW533 | pJC69-P206-gfp-ftsX (lacIq, oriRR6Kγ, attPφ80, Spcr) | This study and ref. 7 |
| pDSW609 | pTH18kr-ftsE-3xHA (Plac, Kanr, pSC101 ori) | This study and ref. 19 |
| pDSW610 | pBAD33-ftsEX | This study |
| pDSW621 | pBAD33-ftsE | This study |
| pDSW622 | pBAD33-ftsX | This study |
| pDSW636 | pDSW209-ftsX | This study |
| pDSW637 | pDSW210-ftsX | This study |
| pDSW638 | pDSW206-ftsE-3xHA | This study |