TABLE 2.
Complementation of the ftsE400::kan allele in RG60a
| Plasmid(s) | Arabinose | Glucose |
|---|---|---|
| pBAD33 | − | − |
| pBAD33-ftsE | − | − |
| pBAD33-ftsX | − | − |
| pBAD33-ftsEX | + | − |
| pBAD33-ftsE + pDSW209 (vector) | − | − |
| pBAD33-ftsE + pDSW636 (gfp-ftsX) | + | − |
| pBAD33-ftsE + pDSW210 (vector) | − | − |
| pBAD33-ftsE + pDSW637 (ftsX-gfp) | + | − |
| pBAD33-ftsX + pDSW206 (vector) | − | − |
| pBAD33-ftsE + pDSW638 (ftsE-3xHA) | + | − |
a
Complementation was determined by colony formation when streaked onto LB plates with no NaCl but containing arabinose or glucose to modulate expression of genes under control of PBAD. IPTG was not necessary for complementation.