TABLE 3.

Localization of GFP-FtsX or FtsX-GFP in fts mutant backgrounds^a

Mutant	Growth condition	No. of cells scored	Avg cell length (μm)	Total no. of FtsX rings	% Cells with a ring(s)	Spacing of ringsb
Wild type	30°C	254	4.5	103	44	10
	42°C	225	7.2	91	40	18
Temperature-sensitive mutants
ftsZ(Ts)	30°C	135	13	36	27	15
	42°C	101	34	0	0	>3,400
ftsA(Ts)	30°C	218	6.3	121	56	11
	42°C	112	36	95c	60	42
zipA(Ts)	30°C	174	4.0	53	30	13
	42°C	105	33	4	3.8	880
Depletion strains
FtsK	Arabinose	208	3.0	112	54	5.7
	Glucose	145	18	261	98	10
FtsQ	Arabinose	187	6.9	123	66	11
	Glucose	132	28	231	91	18
FtsL	Arabinose	220	8.1	157	71	11
	Glucose	72	38	152	83	18
FtsW	Arabinose	265	4.5	113	43	11
	Glucose	61	29	104	89	17
FtsI	Arabinose	212	4.5	111	52	8.6
	Glucose	111	22	230	92	10

^aStrains used were EC1116, EC1158, EC1152, EC1340, EC1295, EC1179, EC1180, EC1159, and EC1181.

^bUnits are micrometer per ring. The spacing is a measure of the frequency of rings per unit cell mass and was calculated by dividing the total number of rings (column 5) into the total length of cells or filaments scored (column 3 multiplied by column 4).

^cThese bands were faint, and so the numbers given understate the degree of dependence on FtsA.